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. 2020 Feb 4;10:1759. doi: 10.1038/s41598-020-58576-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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16S rRNA-based OTU diversity indices. (A) OTU richness. (B) Chao 1 richness estimate. (C) Shannon-Wiener index of diversity. (D) Phylogenetic diversity based on PD whole tree. Values with different superscript letters are significantly different (Wald chi-square test, P < 0.05) based on the interaction effect in a mixed linear model. The main effect “site” is significant (Wald chi-square test, P < 0.05) for OTU richness, Chao1 and phylogenetic diversity. Differences between reference and reclamation area (pair-wise comparison) for each site is not significant (Mann-Whitney U, Test, P < 0.05).