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. 2020 Feb 4;10:1759. doi: 10.1038/s41598-020-58576-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bray-Curtis dissimilarity between bacterial communities (97% 16S rRNA gene similarity). (A) Non-metric dimensional scaling (nMDS) plot. (B). UPGMA hierarchical cluster dendrogram. Dotted lines in the nMDS plot show the distance of every sample to its group centroids in multivariate space, while ellipses show 95% confidence intervals (standard error) in multivariate space around group centroids. The stress of the nMDS plot is 0.067 (also see Fig. S5). Differences in multivariate space are significant for site and history interactions (PERMANOVA R² = 14.8%, P = 0.001). See Table S3 for pair-wise PERMANOVA test for reference and reclamation soils per site. nMDS and UPGMA cluster dendrograms were constructed by using the vegan (v. 2.5.5) and dendextend (v. 1.12.0) packages of R software (https://cran.r-project.org/), respectively.