Fig. 1.

CCR2 deficiency leads to reduced CD25 expression and loss of CD25⁺ FoxP3⁺ Tregs. Tissues were harvested from WT and CCR2−/− mice (five each; 9-week-old male mice) for analysis. a Representative plots showing the expression of FoxP3 and CD25 on gated CD4⁺ T cells from different tissues. b Representative plots showing the expression of FoxP3 and CD25 on gated FoxP3⁺CD4⁺ T cells from different tissues. c Bar graphs showing the levels of CD25 expression (as the mean fluorescence index (MFI)) by gated FoxP3⁺ T cells. d Bar graphs showing the percentages of cells with high CD25 expression among FoxP3⁺ T cells. e, f. Bar graphs showing the percentages and numbers of FoxP3⁺ cells. The data shown are the mean ± SEM of four individual animals. All data were analyzed using two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P<0.0001. Five repeated experiments showed similar results