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. 2020 Jan 20;52(1):41–55. doi: 10.1038/s12276-019-0356-6

Fig. 4. USP21 plays a role as an oncogene through Yin Yang-1 (YY1).

Fig. 4

a Relative YY1 mRNA expression after upregulating or silencing of USP21. b Western blot analyses of YY1 expression after upregulating or silencing of USP21. β-Actin was used as an internal control. c Cell proliferation using the MTT assay at 24, 48, 72, and 96 h in A549 cells transfected with si-USP21- and/or YY1-overexpressing plasmid. d, e Migration and invasion assays in A549 cells transfected with si-USP21- and/or YY1-overexpressing plasmid. Representative images (left panel) and quantifications (right panel) are shown. Scale bar = 100 μm. f The tumor weights and volumes from untransfected H460 cells (control), H460 cells transfected with si-USP21 or YY1, and H460 cells cotransfected with si-USP21 and YY1. n = 5 mice per group. Error bars: mean ± SD. n = 3. **p < 0.01 vs. the corresponding control; #p < 0.05, ##p < 0.01 vs. the si-USP21 group.