Table 1.

List of servers used in the computational analysis:

Si No:	Name of web server	Function	Reference	Submission parameters
1	mCSM	Predict protein stability changes due to mutations.	[28]	Model PDB file, mutation and chain id.
2	SDM	Predict protein stability changes due to mutations.	[18]	Model PDB file, mutation and chain id.
3	mCSM-PPI	Predict stability of protein–protein interfaces due to mutations.	[28]	Model PDB file, mutation and chain id.
4	mCSM-NA2	Predict stability of protein-nucleic acid interactions due to mutations	[29]	Model PDB file, mutation, chain id and nucleic acid type.
5	mCSM-lig	Stability of protein–ligand interactions due to mutations	[30]	Model PDB file, mutation, chain id, three letter code of the ligand and ligand affinity in wild type structure in nM concentration.
6	FoldX4	Predict protein stability changes due to mutations.	[19]	Model PDB file, list of mutations and chain ids.
7	MAESTRO	Predict protein stability changes due to mutations.	[31]	Model PDB file, list of mutations and chain ids.
8	CUPSAT	Predict protein stability changes due to mutations.	[32]	Model PDB file, list of mutations and chain ids.
9	Imutant 2.0-Struc	Predict protein stability changes due to mutations.	[33]	Model PDB file, list of mutations and chain ids.
10	Imutant 2.0 -Seq	Predict protein stability changes due to mutations using sequence information.	[33]	RNAP sequence file in fasta format, list of mutations and chain ids.
11	PROVEAN	Predict protein stability changes due to mutations using sequence information.	[34]	RNAP sequence file in fasta format, list of mutations and chain ids.
12	CONSURF	To calculate evolutionary conservation score of each residue in the protein.	[35]	Model PDB file
13	ENCoM	Conformational Changes in protein due to mutations.	[20]	Model PDB file, list of mutations and chain ids.
14	DynaMut	Conformational Changes in protein due to mutations.	[21]	Model PDB file, list of mutations and chain ids.
15	Arpeggio	Map interatomic interactions between wildtype and mutant amino acids and the residue environment.	[36]	Model PDB file and the residue selection in standard format.
16	Intermezzo	Map interatomic interactions between wildtype and mutant amino acids and the residue environment.	Bernardo Ochoa Montano & Blundell TL unpublished	Model PDB file and the residue selection in standard format.
17	ANDANTE	Works along with Modeller to generate mutant models from wildtype model files.	[26]	Model PDB file, mutation and chain id
18	Fragment Hotspot Maps	Maps regions on the surface of the protein that has high propensity for small molecule binding.	[22]	Model PDB file.