Skip to main content
. 2020 Jan 29;10:1658. doi: 10.3389/fphar.2019.01658

Figure 2.

Figure 2

N1-like and N2-like neutrophils had different effects on HL-60 cells. (A) Cell autophagy as indicated by LC-3B and P62 staining (n = 5, 40×). (B, C) and (C) Cell apoptosis detected by the Annexin V-FITC apoptosis kit (n = 5). (D) HL-60 cell viability detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (n = 5). (E) Cytolytic activity (n = 5). The data present mean ± SD, the experiments were repeated three times, and statistical significance was determined by a t-test. (A, C, D) *P < 0.05, **P < 0.01 vs. HL60; ##P < 0.01 vs. HL60-N2. (E) *P < 0.05, **P < 0.01 vs. HL60-N1; ##P < 0.01 vs. HL60-N2. DOX, doxorubicin; BER, berberine.