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. 2020 Jan 29;10:1658. doi: 10.3389/fphar.2019.01658

Figure 5.

Figure 5

Neutrophils affect urethane-induced lung carcinogenesis. (A) Alveolar neutrophils indicated by hemotoxylin and eosin (H&E) staining of lung tissue (n = 5, 100×). (B) The number of lung nodes (n = 10). (C) Alveolar neutrophil protein expression of CD66b, CD309, and CD133 indicated by immunofluorescence (n = 5, 40×). (D) NLR in peripheral blood (n = 5). (E) Serum PD-L1 levels (n = 5). (F) Spleen tissue PD-1 expression examined by immunohistochemical analysis (n = 5, 100×). (G) Lung tissue protein expression of CD31, tie-2, and CD309 indicated by immunofluorescence (n = 5, 40×). (H) Lung tissue permeability to Evans blue (n = 5). (I) Spleen cytotoxic CD8+ cell rate (n = 5). The data present mean ± SD, the experiments were repeated three times, and statistical significance was determined by a t-test. *P < 0.05, **P < 0.01 vs. control. DOX, doxorubicin; BER, berberine.