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. 2020 Jan 24;11:114–119. doi: 10.1016/j.ijppaw.2020.01.011

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Coccidia-specific PCR to confirm the presence of DNA of various parasite species used to test the analytic specificity of the BdanjoRT1 real-time PCR. (1) Besnoitia darlingi, (2) B. neotomofelis, (3) B. oryctofelisi, (4) B. besnoiti (Evora isolate), (5) B. bennetti (Texas), (6) B. tarandi (Bt-CA-Quebec1), (7) Toxoplasma gondii, (8) Hammondia hammondi, (9) Neospora caninum, (10) H. heydorni, (11) Cystoisospora felis, (12) C. rivolta, (13) C. burrowsi, (14) C. canis, (15) Sarcocystis cruzi and (16) Crytosporidium parvum. Presence of (17) Giardia duodenalis and (18) Tritrichomonas foetus DNA was shown by amplification using species or genus-specific primers, respectively. C, negative control; M, marker.