Table 1.

Primers, probes and their final concentrations in a novel real-time PCR assay (named BdanjoRT1), established to amplify DNA of Besnoitia darlingi, B. jellisoni, B. akodoni, B. neotomofelis and B. oryctofelisi.

Assay	Names of primers and probes	Sequences of primers and probes 5′–3′	Modifications of probes	Final concentration	Reference
BdanjoRT1
	BdanjoFor	CAA CCA TTC AAC CTT TGA ACC C		500 nM	This study
	BdanjoRev	CAC CAT ACT TCC CGA ATG CAC		500 nM	This study
	Bb11-12	CCC TCG AAA CGA GAG ATG CAA GC	5′-FAM, 3′-BHQ1	100 nM	Schares et al. (2011)
Internal control PCR, IC2 PCR
	EGFP1-F	GAC CAC TAC CAG CAG AAC AC		500 nM	Hoffmann et al. (2006)
	EGFP2-R	GAA CTC CAG CAG GAC CAT G		500 nM	Hoffmann et al. (2006)
	EGFP1	AGC ACC CAG TCC GCC CTG AGC A	5′-HEX, 3′-BHQ1	160 nM	Hoffmann et al. (2006)