Table 3.
Significant differences induced by FOS treatment on bacterial Phyla, Families and Genera
| 0 h | 24 h | ∆ | Effect | P | ||||
|---|---|---|---|---|---|---|---|---|
| Cellulose (%) | FOS (%) | Cellulose (%) | FOS (%) | Cellulose | FOS | |||
| Phylum | ||||||||
| Proteobacteria | 3.11 (0.312) | 2.881 (0.279) | 11.082 (1.226) | 3.968 (1.628) | + 7.972 (1.368) | + 1.087 (1.904) | ↓ | 0.043 |
| Family | ||||||||
| Streptococcaceae | 0.067 (0.043) | 0.129 (0.045) | 0.097 (0.056) | 0.003 (0.003) | + 0.03 (0.014) | − 0.126 (0.043) | ↓ | 0.025 |
| Clostridium XIII IS | 0.032 (0.013) | 0.011 (0.011) | 0.069 (0.067) | 0.227 (0.063) | + 0.037 (0.016) | + 0.216 (0.053) | ↑ | 0.032 |
| Lachnospiraceae | 32.303 (2.438) | 27.771 (0.886) | 16.718 (0.485) | 21.909 (1.271) | − 15.585 (1.99) | − 5.862 (0.387) | ↑ | 0.009 |
| Alcaligenaceae | 0.636 (0.088) | 0.988 (0.262) | 6.444 (0.569) | 1.959 (0.612) | + 5.807 (0.612) | + 0.971 (0.848) | ↓ | 0.010 |
| Genus | ||||||||
| Butyricimonas | 0.072 (0.029) | 0.187 (0.046) | 0.288 (0.081) | 0.079 (0.023) | + 0.217 (0.094) | − 0.109 (0.032) | ↓ | 0.030 |
| Parabacteroides | 0.488 (0.152) | 0.735 (0.11) | 1.293 (0.35) | 0.579 (0.11) | + 0.805 (0.255) | − 0.155 (0.084) | ↓ | 0.023 |
| Streptococcus | 0.06 (0.041) | 0.129 (0.045) | 0.097 (0.056) | 0.003 (0.003) | + 0.037 (0.015) | − 0.125 (0.043) | ↓ | 0.023 |
| Christensenella | 0.058 (0.019) | 0.041 (0.015) | 0.094 (0.027) | 0.033 (0.005) | + 0.037 (0.009) | − 0.007 (0.01) | ↓ | 0.033 |
| Clostridium XIII IS UC | 0.012 (0.012) | 0.008 (0.008) | 0.023 (0.006) | 0.212 (0.052) | + 0.013 (0.016) | + 0.204 (0.045) | ↑ | 0.016 |
| Flavonifractor | 0.085 (0.017) | 0.079 (0.004) | 0.282 (0.032) | 0.082 (0.006) | + 0.197 (0.046) | + 0.003 (0.008) | ↓ | 0.047 |
| Sutterella | 0.451 (0.046) | 0.832 (0.302) | 5.304 (1.0) | 1.569 (0.471) | + 4.85 (0.989) | + 0.737 (0.772) | ↓ | 0.030 |
| Cronobacter | 0 (0) | 0.003 (0.003) | 0.116 (0.023) | 0.02 (0.016) | + 0.116 (0.023) | + 0.017 (0.012) | ↓ | 0.019 |
An overview of the significant changes in the relative abundance of the Phyla, Family and Genus (as determined by 454 pyrosequencing of the 16S rDNA region) of the batch culture fermentate following a 24-h incubation with 1% (w/v) FOS or cellulose under anaerobic conditions at 37 °C. Significant differences of delta change values (∆) (difference 0 and 24 h) between FOS and cellulose were determined using independent t tests (n = 3). Data presented are mean (SEM)