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List of primers and probes used for ddPCR copy number estimation
| Primer name | Primer sequence (5′ → 3′) | Amplicon size (bp) | Probe sequence |
|---|---|---|---|
| ddgfp-F | GCACAAGCTGGAGTACAACTA | 98 | 5′/HEX/AGCAGAAAGA/ZEN/ACGGCATCAAGGTGA/3IABkFQ3′ |
| ddgfp-R | TGTTGTGGCGGATCTTGAA | ||
| mcm-F | AGATGCTATAGATGCCCATAAC | 100 | 5′/56-FAM/CCAGGCACA/ZEN/ATAGTCTGAACCAGGT/3IABkFQ3′ |
| mcm-R | GGAGAGCTGCCAAGAAATA |
