Table 3.
Transgene copy number estimation in excised and unexcised events using ddPCR
Agrobacterium strain/banana cultivar | Number of copies | Number of lines |
---|---|---|
LBA4404:Grand Naine unexcised | 1 | 6 |
2 | 23 | |
3 | 0 | |
More than 3 | 0 | |
Average = 2 | Total = 29 | |
AGL1:Grand Naine unexcised | 1 | 4 |
2 | 2 | |
3 | 0 | |
More than 3 | 20 | |
Average = 13 | Total = 26 | |
LBA4404:Williams unexcised | 1 | 21 |
2 | 0 | |
3 | 7 | |
More than 3 | 2 | |
Average = 2 | Total = 30 | |
AGL1:Williams unexcised | 1 | 8 |
2 | 6 | |
3 | 4 | |
More than 3 | 11 | |
Average = 5 | Total = 29 | |
LBA4404:Grande Naine excised | 1 | 5 |
2 | 0 | |
3 | 0 | |
More than 3 | 0 | |
Average = 1 | Total = 5 | |
AGL1:Grand Naine excised | 1 | 1 |
2 | 2 | |
3 | 1 | |
More than 3 | 1 | |
Average = 3 | Total = 5 | |
LBA4404:Williams excised | 1 | 2 |
2 | 3 | |
3 | 0 | |
More than 3 | 0 | |
Average = 2 | Total = 5 | |
AGL1:Williams excised | 1 | 4 |
2 | 1 | |
3 | 0 | |
More than 3 | 0 | |
Average = 1 | Total = 5 |