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. 2019 Oct 22;29(1):53–68. doi: 10.1007/s11248-019-00177-8

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Tamoxifen-independent activation of the Ai14 reporter in primary brain EC isolated from Cdh5(PAC)CreER^T2 mice. a Confocal images of primary brain endothelial cells. Left panel: clusters of EC isolated from Tamoxifen-induced Cdh5(PAC)Cre-ER^T2/Ai14 animals exhibiting a high percentage of tdTomato expressing cells. Middle panel: EC clusters from Tamoxifen-induced mice carrying only the Ai14, but not the Cdh5(PAC)-CreERT2 construct do not show tdTomato expression. Right panel: endothelial clusters from animals that were not Tamoxifen induced but carry both Cdh5(PAC)-CreERT2 and Ai14 show several tdTomato positive cells and reveal a low level of basal Cre activity. Scale bars: 75 μm. b Quantification of the percentage of tdTomato positive cells in cultures of non-Tamoxifen-induced Cdh5(PAC)Cre-ERT2/Ai14 animals one and three days after isolation. ***P ≤ 0.001