Table 1.
SCFA and lactate concentration in batch cultures at 0, 4, 8 and 24 h fermentation comparing no substrate, FOS, AGP and AX
| Time | Lactate | Acetate | Propionate | Butyrate | Total | |
|---|---|---|---|---|---|---|
| Negative control | 0 | 2.28 (1.20) | 3.13 (1.05) | 3.52 (0.62) | 2.86 (1.08) | 21.61 (1.48) |
| 8 | 6.30 (1.79) | 8.31 (1.84) | 5.10 (0.97) | 1.57 (0.65) | 27.12 (5.41) | |
| 24 | 10.17 (2.42) | 8.28 (2.04) | 10.25 (5.19) | 3.32 (1.24) | 36.33 (8.49) | |
| FOS | 0 | 7.22 (3.51) | 9.16 (1.09) | 5.27 (1.80) | 2.33 (0.28) | 27.55 (8.28) |
| 8 | 12.61 (8.95) | 30.57 (4.77)* | 11.27 (5.59) | 3.09 (1.98) | 67.45 (17.12)* | |
| 24 | 10.16 (5.53) | 33.35 (6.69)* | 13.03 (7.03) | 8.36 (6.59) | 72.36 (32.92)* | |
| AGP | 0 | 7.86 (5.12) | 6.80 (3.25) | 3.49 (1.52) | 5.78 (1.67) | 25.54 (5.97) |
| 8 | 5.75 (2.37) | 23.83 (5.95)* | 12.33 (5.09) | 2.81 (1.07) | 50.96 (11.40)* | |
| 24 | 3.48 (1.65)* | 39.34 (4.53)* | 8.60 (6.90) | 5.20 (3.53) | 61.95 (9.94)* | |
| AGP + AX | 0 | 10.01 (2.58) | 6.95 (3.49) | 5.48 (1.37) | 3.53 (0.84) | 36.92 (9.28) |
| 8 | 4.50 (1.98) | 17.05 (8.52) | 12.86 (5.25) | 2.37 (1.94) | 44.08 (15.81) | |
| 24 | 2.38 (1.03)* | 38.91 (9.58)* | 15.61 (6.47) | 2.95 (1.74) | 67.42 (10.47)* | |
| Negative control for AX | 0 | 11.02 (3.68) | 4.21 (1.22) | 4.36 (1.11) | 2.22 (0.50) | 21.85 (3.15) |
| 8 | 5.66 (0.44) | 9.05 (2.40) | 8.77 (1.42) | 1.53 (0.36) | 26.24 (3.97) | |
| 24 | 6.45 (0.16) | 9.84 (2.71) | 9.33 (1.85) | 1.20 (0.22) | 30.08 (1.97) | |
| AX | 0 | 16.39 (3.37) | 4.93 (1.01) | 5.05 (0.60) | 1.54 (0.20) | 27.91 (3.04) |
| 8 | 10.19 (2.86) | 21.12 (4.46)* | 8.09 (0.48) | 1.76 (1.76) | 47.79 (4.99)* | |
| 24 | 2.56 (1.31)* | 27.64 (4.85)* | 9.76 (2.77) | 1.29 (0.12) | 57.15 (12.25)* |
Formate is included in total SCFA but not shown. One-way AVONA was applied to the data to test the main interaction between treatments. Significant interaction between treatments and negative control are denoted. Standard error of the mean (SEM) is shown in brackets. Significant differences between treatments and relevant negative control are denoted *p = 0.05 (F test)