Table 2.
Bacterial enumeration of in vitro batch culture fluid after fermentation comparing no substrate, FOS, AGP and AX
| Time (h) | Bifidobacterium genus | Lactobaccillus Enterococcus group | Bacteroides- Prevotella group | Clostridium coccoides- Eubacterium rectale group | Roseburia | Atopobium cluster | Clostridium cluster IX | Faecalibacterium prausnitzii group | Desulfovibrionales | Clostridium-cluster I and II | Total | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Negative | 0 | 7.96 (0.26) | 6.80 (0.38) | 7.14 (0.031) | 8.53 (0.33) | 6.78 (0.75) | 7.20 (0.58) | 7.35 (0.36) | 8.28 (0.25) | 7.85 (0.47) | 6.86 (0.40) | 8.97 (0.33) |
| 8 | 7.72 (0.11) | 7.16 (0.12) | 7.43 (0.22) | 8.37 (0.25) | 7.38 (0.02) | 7.20 (0.10) | 7.42 (0.27) | 8.06 (0.24) | 7.69 (0.30) | 7.31 (0.17) | 8.85 (0.22) | |
| 24 | 7.68 (0.66) | 7.25 (0.73) | 7.36 (0.68) | 8.00 (0.49) | 7.46 (0.71) | 7.51 (0.52) | 7.53 (0.60) | 7.49 (0.58) | 7.38 (0.46) | 7.12 (0.73) | 8.62 (0.57) | |
| FOS | 0 | 7.88 (0.27) | 7.01 (0.46) | 7.57 (0.43) | 8.23 (0.27) | 7.41 (0.50) | 7.01 (0.49) | 7.45 (0.35) | 8.22 (0.25) | 7.75 (0.46) | 7.11 (0.59) | 8.87 (0.30) |
| 8 | 9.83 (0.15)* | 7.32 (0.60) | 8.20 (0.40) | 9.70 (0.17)* | 8.16 (0.70) | 8.02 (0.46) | 8.22 (0.38) | 8.31 (0.41) | 7.88 (0.55) | 7.69 (0.48) | 10.26 (0.09)* | |
| 24 | 8.95 (0.21) | 7.13 (0.72) | 7.43 (0.66) | 8.73 (0.20) | 7.29 (0.72) | 7.17 (0.54) | 7.72 (0.60) | 7.89 (0.58) | 7.61 (0.60) | 7.16 (0.60) | 9.36 (0.27) | |
| AGP | 0 | 7.96 (0.25) | 7.02 (0.26) | 7.23 (0.10) | 8.59 (0.19) | 7.66 (0.24) | 7.27 (0.30) | 7.41 (0.17) | 8.19 (0.24) | 8.04 (0.19) | 7.16 (0.19) | 9.00 (0.20) |
| 8 | 8.89 (0.45) | 6.87 (0.51) | 6.74 (0.70) | 8.92 (0.36) | 7.89 (0.22) | 7.23 (0.14) | 7.32 (0.30) | 8.47 (0.16) | 8.06 (0.08) | 7.18 (0.35) | 9.49 (0.31) | |
| 24 | 9.39 (0.78)* | 7.26 (0.33) | 7.68 (0.83) | 9.28 (0.47)* | 7.46 (0.30) | 7.26 (0.37) | 7.89 (0.78) | 8.03 (0.17) | 7.19 (0.35) | 6.24 (0.76) | 9.84 (0.55)* | |
| AGP + AX | 0 | 7.98 (0.18) | 7.06 (0.36) | 7.20 (0.46) | 8.63 (0.29) | 7.37 (0.46) | 7.12 (0.46) | 7.20 (0.41) | 8.38 (0.19) | 7.95 (0.36) | 7.04 (0.40) | 9.06 (0.26) |
| 8 | 9.35 (0.46)* | 7.22 (0.44) | 7.74 (0.28) | 9.36 (0.38)* | 7.61 (0.78) | 7.00 (0.12) | 7.63 (0.31) | 8.59 (0.23) | 8.00 (0.35) | 6.94 (0.30) | 9.89 (0.33)* | |
| 24 | 8.82 (0.28) | 6.88 (0.76) | 6.73 (0.67) | 8.82 (0.19) | 6.64 (0.75) | 6.27 (0.28) | 6.47 (0.53) | 7.13 (0.30) | 6.07 (0.45) | 5.62 (0.43) | 9.25 (0.13) | |
| Negative for AX | 0 | 8.53 (0.33) | 8.76 (0.13) | 7.95 (0.12) | 9.11 (0.04) | 8.63 (0.11) | 7.60 (0.16) | 8.20 (0.09) | 9.07 (0.04) | 8.61 (0.06) | 7.75 (0.15) | 9.70 (0.03) |
| 8 | 8.58 (0.32) | 7.65 (0.03) | 8.55 (0.03) | 9.01 (0.08) | 8.25 (0.13) | 7.70 (0.09) | 8.70 (0.06) | 8.90 (0.02) | 8.54 (0.10) | 8.20 (0.12) | 9.67 (0.07) | |
| 24 | 8.76 (0.3) | 7.90 (0.18) | 8.45 (0.13) | 8.92 (0.07) | 7.74 (0.20) | 7.85 (0.10) | 8.76 (0.08) | 8.54 (0.06) | 8.12 (0.12) | 7.93 (0.12) | 9.58 (0.10) | |
| AX | 0 | 8.52 (0.41) | 7.92 (0.14) | 8.11 (0.17) | 9.13 (0.01) | 8.65 (0.06) | 7.88 (0.05) | 8.28 (0.12) | 8.89 (0.09) | 8.58 (0.03) | 8.00 (0.16) | 9.67 (0.07) |
| 8 | 9.23 (0.19)* | 7.67 (0.20) | 8.84 (0.23) | 9.09 (0.38) | 8.32 (0.52) | 7.65 (0.37) | 8.93 (0.24) | 9.00 (0.19) | 8.61 (0.19) | 7.81 (0.40) | 9.94 (0.24)* | |
| 24 | 9.84 (0.09)* | 8.08 (0.22) | 8.55 (0.44) | 9.57 (0.18)* | 8.42 (0.40) | 8.45 (0.53) | 8.63 (0.44) | 9.06 (0.17) | 8.26 (0.20) | 8.11 (0.42) | 10.29 (0.05)* |
Negative control is no added carbohydrate and positive control is FOS. Values are mean log10 bacterial numbers/mL found using flow FISH. One-way AVONA was applied to the data to test the main interaction between treatments. Values in brackets are SEM. Significant difference between treatments and relevant negative control are denoted *p = 0.05 (F test)