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. 2020 Jan 29;10:1764. doi: 10.3389/fpls.2019.01764

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Copyright © 2020 Zhao, Wang, Fu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The relationship between ΔCt value and cDNA fragment size analyzed using the same genes. cDNAs of control (100% germination), AA (artificially aged, 0.5% germination) and NA (naturally aged, 0.5% germination) seeds were used to analyze different fragments of the B16 and B20 genes. Each ΔCt value was based on three biological repeats. (A) Correlation between the ΔCt value and cDNA fragment length for six cDNA fragments (starting from the STOP codon) of the gene B16. (B) Correlation between the ΔCt value and cDNA fragment length for five cDNA fragments (starting from the STOP codon) of the gene B20. Linear regressions were obtained with Microsoft Excel 2010 and all have a P <0.0001. ▲: ΔCt values for AA seeds, ▪: ΔCt values for NA seeds.