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. 2020 Jan 29;10:1764. doi: 10.3389/fpls.2019.01764

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Copyright © 2020 Zhao, Wang, Fu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Percentage of estimated undamaged mRNAs of B16 and B20 in seeds with different artificial aging time (days). We have derived mathematical equations to estimate the relative amount or percentage of undamaged stored mRNAs based on the ΔCt regression functions. The regression functions for different fragments of B16 and B20 are from Figures 3C, D . For B162,000/250bp, the percentages at different aging times were calculated based on the equation: y = 1/2ΔCt(B162,000/250bp) × 100% = 1/2(0.301+ 0.082)100%, while for B202,000/250bp, the equation is y = 1/2ΔCt(B202,000/250bp) × 100% = 1/2(0.252+ 0.158) 100%.