Table 5.
Evaluation of biosurfactant production by Halomonas Pacifica strain Cnaph3 growing on various carbon sources at 37 °C and 180 rpm during 10 days
| Carbon sources | ODmax (600 nm) | STI (mN/m) | STF (mN/m) | RST (mN/m) | ODT (cm) | Biosurfactant yield (g/L) |
|---|---|---|---|---|---|---|
| Nutrient Broth (NB) | 1.78 ± 0.1**** (2d) | 59.3 ± 0.4 | 27 ± 0.06**** | 32.3 ± 0.53**** | 7.8 ± 0.24**** | 1.21 ± 0.08**** |
| Olive oil (1%, v/v) | 1.66 ± 0.1**** (2d) | 56.2 ± 0.7*** | 31.3 ± 0.64**** | 24.9 ± 1.42**** | 7.9 ± 0.35**** | 0.85 ± 0.03**** |
| Corn oil (1%, v/v) | 1.35 ± 0.08**** (2d) | 57.5 ± 0.5** | 32.2 ± 0.57**** | 25.2 ± 0.08**** | 7.8 ± 0.15**** | 0.81 ± 0.06**** |
| Glycerol (1%, v/v) | 1.26 ± 0.15**** (5d) | 54.5 ± 0.4**** | 27.2 ± 0.17**** | 27.3 ± 0.26**** | 8.5 ± 0.06**** | 0.75 ± 0.03**** |
| Diesel oil (1%, v/v) | 0.31 ± 0.07 (3d) | 57 ± 0.7** | 42.6 ± 0.46**** | 14.4 ± 1.17**** | 4.1 ± 0.13**** | 0.12 ± 0.006 |
| Motor oil (1%, v/v) | 0.23 ± 0.07 (3d) | 55.1 ± 0.5**** | 43.9 ± 0.62**** | 11.2 ± 0.8**** | 3.7 ± 0.51**** | 0.11 ± 0.004 |
| Crude oil (1%, v/v) | 0.31 ± 0.05 (4d) | 55.4 ± 0.5**** | 42.9 ± 1.46**** | 12.5 ± 1.68**** | 3.6 ± 0.5**** | 0.13 ± 0.01 |
| Biotic control | 0.14 ± 0.02 (2d) | 61.4 ± 1.6 | 59.2 ± 0.17 | 2.16 ± 1.44 | 0.6 ± 0.06 | 0 |
Values given represent the mean of three replicates ± standard deviation
ODmax maximum optical density, d days, STI initial surface tension, STF final surface tension, RST reduction of surface tension, ODT oil displacement test
*, **, ***, ****Significant differences (p < 0.05) versus biotic control (**** < 0.0001; *** < 0.001; ** < 0.01 and * < 0.05)