Index of inner cell mass (ICM):trophectoderm (TE) in blastocysts. Staining was performed by immunostaining for CDX2, a specific marker of TE (green), and DAPI, to stain the nuclei of all blastomeres (blue). (B) Quantification of the ICM/TE index of blastocysts in the IVF, ICSI-C, and ICSI-T groups. Data are shown as the mean ± SEM. Values with different superscripts differ significantly (P < 0.05)