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Real time measurement of SenVitAL-5 in mammalian cells. a Confocal image of HEK-293T cell expressing the nanosensor showing the channels: ECFP, venus, merge and ratiometric (Scalebar–3 μm). b Change in the emission intensities of the fluorophores ECFP and venus with addition of vitamin B12. c Time-dependent FRET ratio change in the presence of externally supplied 50 µM vitamin B12. The graph indicating the venus/ECFP ratio change for 10 min
