Table 1.

Binding constants and dynamic range of SenVitAL-WT and mutants

Sensor namea	Sequences	Kd (M) (± SD)	Dynamic rangeb (µM)
SenVitAL-93	WT	93.36 × 10–6 (± 0.02)	0.53–360
SenVitAL-5	W44Q	5.04 × 10–6 (± 0.03)	0.10–480
SenVitAL-143	P144G	143.40 × 10–6 (± 0.02)	5.00–300
SenVitAL-126	A225P	126.27 × 10–6 (± 0.01)	0.78–400

Binding constants was determined in vitro

SD standard deviation

^aNumber next to the mutant name stands for the K_d of the sensor variants

^bEffective quantification range between 10 and 90% saturation of the nanosensor