Table 1.
Primers used to determine the relative expression of corticosteroid signaling pathway genes and housekeeping genes by RT-qPCR and to quantify DNA immunoprecipitation of Gilz by MeDIP.
| Name | Forward primer | Reverse primer | |
|---|---|---|---|
| Gene expression (RT-qPCR) | 36B4 | AGCGCGTCCCATTGTCTGT | GGGCAGCAGTGGTGGCAGCAGC |
| βactin | AAGTACCCCATTGAACATGCGA | CATCTTTTCACGGTTGGCCTTA | |
| HPRT1 | AGACTTGCTCGAGATGTCATGAAG | AATCCAGCAGGTCAGCAAAGA | |
| 18s | CCGTGCCCTTTGTACACACC | CGATCCGAGGGCCTCA | |
| Nr3C2 | ATGGAAACCACACGGTGACCT | AGCCTTATCTCCACACACCAAG | |
| Nr3C1 | TTCTGTTCATGGCGTGAGTACC | CCCTTGGCACCTATTCCAGTT | |
| αENaC | GGAGTCGAAAATCGGCTTCC | TAGAGCAGGCGAGGTGTCG | |
| Sgk1 | TCACTTCTCATTCCAGACCGC | ATAGCCCAAGGCACTGGCTA | |
| Gilz | CTGCTGTGGAGTTTGTGACATACTAG | CCAGGCAGGCACTTCTAAGCT | |
| Methylation DNA immunoprecipitation-qPCR | Gilz Primer 1 | GAAAACTCAGCCCTTGCTATGG | AAAGCCAAGCAAACCAAACAA |
| Gilz Primer 2 | CCACTTTCCTGCCCAACAAA | GAAGGAGGGAAGCAAGAAGACA | |
| Gilz Primer 3 | CCCTGTGTTTTGCTGGCAATA | ACACTTGAAGCATTGTGTACCACAT | |
| Gilz Primer 4 | TTGCACAGGACACAAGAATATATATGAT | GCTCAAAATAGTTGCACGAAACC | |
| Gilz Primer 5 | GACTTGTCTAAGTATGGGTTGAATCTACA | GGAGCAAGCTTATACCAGGAAGTT | |
| Gilz Primer 6 | CCCATAGTTAGTATGTCATTGATGGAA | CCACGAGGTTGCATTGAATAATAA | |
| Gilz Primer 7 | CCCACCATCTCCCTTGGAAT | CCCTCTGCCACCTAGAGCTTT | |
| Gilz Primer 8 | CAGATAACACTCCCGACGACCTA | TGCCAACCTCTGGACATTTTAA | |
| Gilz Primer 9 | ATTCCTTTTTCTGCCCATGCT | AAAGAAGGCGGCATCTAAGACTT | |
| Gilz Primer 10 | GGATGGAGGTTCTCTTTGGATTC | CGTGCTGATAACAGCTCCATCT | |
| Gilz Primer 11 | GAGGTAGCTCAGCGGCAAGA | ACTGTACCACATGAGTGCCTTGTT | |
| Gilz Primer 12 | AGTTGGCTGGAGAAAGTGAAGAA | GGGCGGTACTGCATTTAAAAGT |