Table 3.
Kinetic parameters.
| l-Glu production | d-GlcN6P production | |||||
|---|---|---|---|---|---|---|
| Km l-Gln [mM] | kcat [s−1] | kcat/Km [mM−1 s−1] | Km Frc6P [mM] | kcat [s−1] | kcat/Km [mM−1 s−1] | |
| Wild type | 1.1 ± 0.2 | 3.6 ± 0.2 | 3.3 | 0.08 ± 0.01 | 1.7 ± 0.1 | 21.3 |
| G461E | 0.5 ± 0.1 | 3.1 ± 0.1 | 6.2 | 0.09 ± 0.01 | 2.1 ± 0.1 | 23.3 |
| G451E | 1.3 ± 0.2 | 2.2 ± 0.1 | 1.7 | 0.04 ± 0.01 | 0.8 ± 0.04 | 20.0 |
| Q307A | 0.6 ± 0.04 | 2.6 ± 0.04 | 4.3 | 0.15 ± 0.01 | 2.1 ± 0.1 | 14.0 |
| Q307A/G451E | 1.0 ± 0.05 | 2.1 ± 0.03 | 2.1 | 0.05 ± 0.01 | 1.1 ± 0.04 | 22.0 |
Wild type, G461E, and G451E: mean ± SEM, n = 5; Q307A and Q307A/G451E: mean ± SEM, n = 3