Fig. 1.
Immunoblotting evaluation of CK2 and phospho-CDC37 expression in GIST cell lines and GIST-frozen tumours. a CK2 expression levels in GIST cell lines (GIST-T1, GIST882 and GIST430/654) as compared with ovarian cancer (SKOV3, OVCA429 and ES2), non-small-cell lung cancer (PC-9, A549), mesothelioma (MESO924, MESO428 and JMN1B) and liposarcoma (LPS510, LPS141 and LPS141/239) cell lines. Actin stain is a loading control. b Expression of CK2, p-CDC37, KIT and activated p-KIT in GIST biopsies and adjacent non-neoplastic tissue samples. GAPDH stain is a loading control. Note the presence of a presumably cross-reacting, nonspecific band with the p-CDC37 antibody on tissue samples. Immunoblotting experiments were performed from two independent experiments for each sample.