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. 2020 Feb 4;10:1777. doi: 10.1038/s41598-020-58610-6

Figure 2.

Figure 2

PKCα does not regulate Ras or Cdc42 activation during sLTP. (a,c) 2pFLIM images of Ras (a) and Cdc42 (c) activation in PKCα WT and KO neurons at indicated time points. Time courses and quantification of transient (1–3 min) and sustained (10–25 min) spine volume change induced by glutamate uncaging in neurons expressing Ras1 sensor (a) or Cdc42 sensor (c) from PKCα WT and KO littermates. (b,d) Time courses and quantification of transient (1–3 min) and sustained (10–25 min) Ras1 (b) or Cdc42 (d) activation in stimulated spines from WT and PKCα KO littermates. Data are mean ± s.e.m. n(neurons/spines) Ras: n = 8/10 WT, n = 7/8 KO. Cdc42: n = 15/21 WT and n = 7/9 KO *P < 0.05, two-tailed t-test.