Figure 5.

Effect of micromolar myricetin and chloroxine on electrically evoked Ca²⁺ transients. In rat skinned fibers, Ca²⁺ transients were obtained by confocal line scans parallel to the fiber long axis, with corresponding line averaged and normalized rhod-2 fluorescence signals (F/F₀). Representative recordings of Ca²⁺ transients are shown in Supplementary Fig. 7. All solutions contained 1 mM EGTA and 100 nM free Ca²⁺. Cytosolic Ca²⁺ transients were elicited by electrical field stimulation at 1 Hz in the presence of DMSO (control), myricetin or chloroxine. Dotted line represents 0.1% DMSO control (which causes no detectable reduction in EC-coupling). Data presented as means ± SEM, *P < 0.05, n = 6.