Table 2.
Altered platelet proteins between LA+ TE+ patients, LA+ TE− patients and healthy controls analyzed by 2D-DIGE.
| Protein name | Gene name | LA+ TE+ /healthy | LA+ TE+ /LA+ TE− | LA+ TE−/healthy | |||
|---|---|---|---|---|---|---|---|
| Average fold-change | p-value [adjusted] | Average fold-change | p-value [adjusted] | Average fold-change | p-value [adjusted] | ||
| 60 kDa heat shock protein | HSPD1 | 1.05 | 0.1045 | 1.14 | 0.0042 | 0.93 | 0.0825 |
| 78 kDa glucose-regulated proteina | HSPA5 | 1.11 | 0.0047 | 1.19 | 0.0002 | 0.93 | 0.0528 |
| Actin | ACTB | 1.18 | 0.1873 | 0.51 | 0.0276 | 2.31 | 0.0074 |
| Albumina | ALB | 0.92 | 0.1138 | 0.76 | 0.0013 | 1.21 | 0.0271 |
| Apolipoprotein A-I | APOA1 | 0.86 | 0.0097 | 0.90 | 0.1716 | 0.96 | 0.5661 |
| ATP synthase subunit beta | ATP5B | 1.09 | 0.6948 | 1.11 | 0.0321 | 0.98 | 0.0662 |
| Bridging integrator 2 | BIN2 | 0.86 | 0.3233 | 1.29 | 0.0671 | 0.67 | 0.0232 |
| Calreticulina | CALR | 1.10 | 0.0019 | 1.16 | 0.0033 | 0.95 | 0.6012 |
| Chloride intracellular channel protein 1 | CLIC1 | 0.60 | 0.2305 | 0.23 | 0.0033 | 2.67 | 0.0297 |
| Fermitin family homolog 3 | FERMT3 | 0.90 | 0.0180 | 1.07 | 0.4783 | 0.76 | 0.0261 |
| Fibrinogen beta chain | FGB | 1.21 | 0.0097 | 1.01 | 0.8706 | 1.19 | 0.0297 |
| 1.09 | 0.0255 | 1.13 | 0.0177 | 0.97 | 0.5661 | ||
| 1.31 | 0.0112 | 1.29 | 0.1716 | 1.02 | 0.5661 | ||
| 1.27 | 0.0130 | 1.30 | 0.1716 | 0.98 | 0.6012 | ||
| Heat shock protein HSP 90-alpha | HSP90AA1 | 1.10 | 0.0026 | 1.06 | 0.1493 | 1.04 | 0.3496 |
| Integrin alpha-6a | ITGA6 | 0.89 | 0.0097 | 0.85 | 0.0018 | 1.06 | 0.2110 |
| Leukocyte elastase inhibitor | SERPINB1 | 0.90 | 0.0164 | 0.94 | 0.1754 | 0.97 | 0.6012 |
| Microtubule-associated protein RP/EB family member 2a | MAPRE2 | 1.13 | 0.1249 | 0.87 | 0.2678 | 1.31 | 0.0297 |
| Myosin 9a | MYH9 | 0.80 | 0.0676 | 0.63 | 0.0013 | 1.29 | 0.0298 |
| Myosin regulatory light polypeptide 9 | MYL9 | 1.09 | 0.2043 | 1.23 | 0.0248 | 0.88 | 0.2110 |
| Nucleosome assembly protein 1-like 1 | NAP1L1 | 1.02 | 0.5365 | 1.23 | 0.0033 | 0.83 | 0.0232 |
| Proteasome activator complex subunit 1 | PSME1 | 1.17 | 0.0014 | 1.15 | 0.0161 | 1.02 | 0.7921 |
| Protein disulfide isomerase A1a | P4HB | 1.10 | 0.0047 | 1.16 | 0.0016 | 0.95 | 0.2835 |
| Protein disulfide isomerase A6 | PDIA6 | 1.07 | 0.0047 | 1.13 | 0.0043 | 1.00 | 0.5661 |
| Ras-related protein Rab-27B | RAB27B | 0.91 | 0.1723 | 1.12 | 0.1716 | 0.81 | 0.0280 |
| Translationally controlled tumor protein | TPT1 | 1.08 | 0.1362 | 1.17 | 0.0177 | 0.93 | 0.2110 |
| Tropomyosin alpha-3 chain | TPM3 | 0.95 | 0.6979 | 1.28 | 0.0160 | 0.74 | 0.0212 |
| Vinculin | VCL | 1.37 | 0.0115 | 1.45 | 0.0800 | 0.94 | 0.9631 |
| 1.26 | 0.0097 | 1.23 | 0.1716 | 1.03 | 0.6012 | ||
| 1.25 | 0.0014 | 1.32 | 0.0106 | 0.95 | 0.9144 | ||
| 0.75 | 0.0385 | 1.03 | 0.7263 | 0.73 | 0.0585 | ||
Differentially regulated proteins were considered significant when the fold-change differed by at least 10% and the p-value was ≤ 0.05. A fold-change above 1 indicates that the protein is upregulated, whereas a fold-change below 1 indicates that the protein is downregulated in the first stated group. For detailed protein description, refer to Supplementary Table 1
Statistically significant correlations are highlighted in bold
LA lupus anticoagulant, TE thromboembolism
aProteins identified in more than one spot and regulated in the same direction. The standardized abundances of the 2D-DIGE spots were summed and divided by the volume of the summarized IS for normalization. Fibrinogen beta chain and vinculin were not regulated in the same direction; therefore, all isoforms are stated. All proteoforms are listed in Supplementary Table 1