Fig. 8.

ILC3-derived OX40L signaling supports large intestinal homeostasis in immunosufficient mouse. a, b Small and large intestinal LPLs were isolated from littermate Tnfsf4^+/– and Tnfsf4^–/– mice. Expression of CD3, CD4, and Foxp3 was analyzed by flow cytometry. Percentages of Tregs (CD3⁺CD4⁺Foxp3⁺ cells) and CD4⁺non-Tregs (CD3⁺CD4⁺Foxp3^- cells) in live lymphocytes from the large intestine (a) or small intestine (b) are shown. c–e Rag1^–/–Tnfsf4^–/– mice were lethally irradiated and reconstituted with bone marrow mixed at 1:1 ratio from Rag1^–/–Rorc^gfp/gfp mice plus Tnfsf4^+/– or Tnfsf4^–/– mice. Small and large intestinal LPLs were isolated for analysis after 6 weeks. c The protocol for the construction of bone marrow chimeric mice is shown. Percentages of Tregs (CD3⁺CD4⁺Foxp3⁺ cells) and CD4+non-Tregs (CD3⁺CD4⁺Foxp3^- cells) in live lymphocytes from the large intestine (d) or small intestine (e) are shown. a–e Data are representative of two independent experiments. LPLs, lamina propria lymphocytes