Figure 1.

Frequencies of different immune cell subsets among the HBs^lo (<500 IU/ml, designated as lo), HBs^hi (>50,000 IU/ml, designated as hi) and healthy donors (HD). (A) Gating strategies for CD4⁺ and CD8⁺ T cells and their subsets based on CD45RO and CCR7 expression; naïve (CD45RO⁻CCR7⁺), central memory (CM^; CD45RO⁺CCR7⁺), effector memory (EM^; CD45RO⁺CCR7⁻) and terminally differentiated effector memory (EMRA; CD45RO⁻CCR7⁻). Gating strategies for B cells and their subsets based on CD21 and CD27 expression; naïve (CD27⁻CD21⁺), resting memory (RM^; CD27⁺CD21⁺), active memory (AM; CD27⁺CD21⁻) and atypical memory (ATM; CD27⁻CD21⁻). (B) Frequencies of total CD4⁺, CD8⁺ T cells and total B cells among groups. (C) Proportions of the naïve, CM, EM and EMRA subsets of CD4⁺ and CD8⁺ T cells. (D) Proportions of the naïve, RM, AM and ATM subsets of B cells. Each data point represents an individual sample and horizontal line represents the median value. One-way-ANOVA/Kruskal-Wallis with Bonferroni/Dunn’s post-hoc tests for multiple comparison were performed for parametric or non-parametric data respectively. *p < 0.05, **p < 0.005.