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. 2020 Jan 20;52(1):56–65. doi: 10.1038/s12276-019-0357-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Experimental scheme used to determine the paracrine effects of PVCs on the hematopoietic differentiation of hPSCs (CHA15 and iPS-NT4-S1). b Representative bright-field images of colonies on day 17 of hematopoietic differentiation. Scale bar, 50 μm. c Effects of PVC-CM on the production of hematopoietic lineage cells for two different exposure times (days 0–9 and days 9–17). d The total number of CFUs was counted by plating 1 × 10⁴ cells in methylcellulose. e Distribution of CFU subtypes. The bars indicate the mean ± SD; *p < 0.05, **p < 0.01 (control vs. CM 0–9 or CM 9–17). CFU-GM, granulocyte-macrophage.