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. 2020 Feb 4;9(2):10. doi: 10.1038/s41389-020-0189-0

Fig. 5. TGFβ downregulates PRH mRNA levels and prevents the activation of E-cadherin expression by PRH.

Fig. 5

a PNT2-C2 cells and PC3 cells were treated with 5 ng/ml TGFβ for 48 h. qRT-PCR was the used to determine PRH mRNA levels relative to GAPDH mRNA. The data shown are from three independent experiments each performed with triplicate PCR reactions. M + SD, *p < 0.01. b PC3 cells were infected with Ad-GFP or Ad-PRH at MOI 100 for 24 h then treated with 5 ng/ml TGFβ for a further 48 h. Chromatin immunoprecipitation was then performed using IgG or pSMAD3 antibodies followed by quantitative PCR using HHEX primers and Chromosome 18 gene desert primers as negative control. The results shown are from three independent experiments performed with triplicate PCR reactions. M + SD, *p < 0.01. c PC3 cells were infected with Ad-ΔE1 empty virus or Ad-PRH at MOI 100 for 24 h. The cells were then treated with vehicle (V), 5 ng/ml TGFβ (T), or control for 48 h. Western blotting was then performed for Myc-PRH and E-Cadherin with Lamin A/C and β-Actin as loading controls, respectively.