Table 2.
Comparable quantification results of TMT and PRM.
| Protein Accession | Protein description | Comparable group | TMT quantification ratios | PRM quantification ratios |
|---|---|---|---|---|
| XP_012041646.1 | PREDICTED: myosin light chain 4 [Ovis aries] | a | 0.74 | 0.49 |
| b | 0.65 | 0.61 | ||
| c | 0.48 | 0.30 | ||
| XP_014958377.1 | PREDICTED: insulin-like growth factor II isoform X2 [Ovis aries] | a | 0.92 | 0.67 |
| b | 0.65 | 0.42 | ||
| c | 0.60 | 0.28 | ||
| XP_004015439.1 | “PREDICTED: myosin-binding protein C, fast-type isoform X1 [Ovis aries]” | a | 1.96 | 9.15 |
| b | 1.06 | 1.86 | ||
| c | 2.07 | 17.05 | ||
| XP_004018449.1 | “PREDICTED: troponin C, slow skeletal and cardiac muscles [Ovis aries]” | a | 0.70 | 0.64 |
| b | 2.40 | 4.62 | ||
| c | 1.68 | 2.94 | ||
| NP_001155363.1 | “fumarate hydratase, mitochondrial [Ovis aries]” | a | 1.08 | 0.86 |
| b | 1.66 | 1.46 | ||
| c | 1.79 | 1.25 | ||
| XP_014955319.1 | PREDICTED: microtubule-associated protein RP/EB family member 1 | a | 0.83 | 0.54 |
| b | 0.76 | 0.43 | ||
| c | 0.63 | 0.23 | ||
| NP_001135426.1 | “myosin regulatory light chain 2, ventricular/cardiac muscle isoform [Ovis aries]” | a | 0.65 | 0.37 |
| b | 2.05 | 3.27 | ||
| c | 1.33 | 1.22 | ||
| XP_014948822.1 | PREDICTED: titin isoform X5 [Ovis aries] | a | 1.11 | 0.68 |
| b | 1.47 | 1.66 | ||
| c | 1.63 | 1.12 | ||
| XP_004015439.1 | “PREDICTED: myosin-binding protein C, fast-type isoform X1 [Ovis aries]” | a | 1.96 | 9.15 |
| b | 1.06 | 1.86 | ||
| c | 2.07 | 17.05 | ||
| XP_014954142.1 | PREDICTED: LOW QUALITY PROTEIN: myosin-3 isoform X1 [Ovis aries] | a | 0.79 | 0.52 |
| b | 0.52 | 0.25 | ||
| c | 0.41 | 0.13 | ||
| XP_004012685.1 | “PREDICTED: very long-chain specific acyl-CoA dehydrogenase, mitochondrial [Ovis aries]” | a | 1.08 | 0.89 |
| b | 2.08 | 2.68 | ||
| c | 2.25 | 2.39 | ||
| XP_011957551.2 | PREDICTED: LOW QUALITY PROTEIN: alpha-actinin-3 isoform X1 [Ovis aries] | a | 1.66 | 7.50 |
| b | 1.35 | 2.82 | ||
| c | 2.24 | 21.18 | ||
| XP_004008702.1 | PREDICTED: fibrillin-2 | a | 0.92 | 0.73 |
| b | 0.63 | 0.36 | ||
| c | 0.58 | 0.27 | ||
| XP_004010374.1 | PREDICTED: myosin-7 [Ovis aries] | a | 0.91 | 0.68 |
| b | 2.55 | 4.95 | ||
| c | 2.33 | 3.35 | ||
| NP_001177319.1 | glyceraldehyde-3-phosphate dehydrogenase [Ovis aries] | a | 1.11 | 0.93 |
| b | 1.66 | 1.78 | ||
| c | 1.83 | 1.66 | ||
| NP_001159670.1 | four and a half LIM domains protein 1 [Ovis aries] | a | 1.16 | 0.96 |
| b | 2.05 | 2.09 | ||
| c | 2.36 | 2.02 |
(a) D105N vs. D85N, (b) D135N vs. D105N and (c) D135N vs. D85N. The ratios of TMT and PRM quantification are the average protein quantitation at one stage to another one such as the a, b and c. (The detail can be found in Supplementary Table S3).