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. 2020 Feb 4;5:2. doi: 10.1038/s41536-020-0087-2

Fig. 2. The presence of a brain protects against infection-induced death, which is more severe during the first 48 h post infection.

Fig. 2

a Longitudinal assay, scoring survival rates after infection for Control (Ctrl, green), Brainless (BR–, red), Spinal Cord Resection (SC–, orange) and Tailless (Tail–, yellow) embryos at 2–4 h post-surgery (hps; corresponding to st. 26–27), 8 hps (st. 30), 24 hps (st. 35), 36 hps (st. 40), 48 hps (st. 42) and the end stage-48 time point (4 days ps). Data represent the mean and S.D. of three independent replicates. b–f Number (b) and expression pattern (c–f) of apoptotic cells (positively reacted against Cleaved Caspase-3 Asp175 antibody; CC3) in Ctrl (green) and BR– (red) animals at the same longitudinal period than (a). Data represent the mean and S.D. of, at least, ten embryos coming from three different replicates. c–f Lateral views after CC3 immunofluorescence of UTI-infected Ctrl (c, e) and BR– (d, f) embryos at st. 35 (c, d) and st. 40 (e, f). White arrows point CC3-expressing cells; red arrows indicate damage related to the infection along the gut area. Rostral is left and dorsal is up. Scale bar = 250 μm. a, b Two-way ANOVA P < 0.01. P values after post hoc Bonferroni comparisons are indicated as **P < 0.01.