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. 2020 Feb 4;10:1804. doi: 10.1038/s41598-020-58642-y

Figure 5.

Figure 5

TNF-α induces generation of transcription factor p52 and TAZ transcription. (A) TNF-α induces generation of transcription factor p52 from p100 in both MCF7 and MDA-MB-468 cells. Cells were treated with 10 ng/ml TNF-α or 0.1% BSA for 12, 24 and 48 h. TAZ, CYR61 and p100/p52 protein expression levels were determined by WB. (B) The TAZ promoter sequence contains a p52 binding site, as highlighted in the shaded box. (C) TNF-α induces p52 binding to the TAZ promoter in both MCF7 and MDA-MB-468 cells, as determined by chromatin Immunoprecipitation (ChIP) assays. Cells were treated with vehicle (PBS) or TNF-α for 3 h. Immunoprecipitation was performed using anti-p52 antibody. PCR or qPCR were performed to quantify precipitated TAZ promoter using specific primers flanking the p52 binding site. (D).