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| Group | 0–2 days | 3–4 days |
|---|---|---|
| F | Null | FGF2 |
| FB | Null | FGF2+BMP4 |
| BFB | BMP4 | FGF2+BMP4 |
| CF | CHIR99021 | FGF2 |
| CFB | CHIR99021 | FGF2+BMP4 |
| CBFB | CHIR99021+BMP4 | FGF2+BMP4 |
BMP4, bone morphogenetic protein 4; CHIR99021, combination of GSK3β inhibitor; FGF2, fibroblast growth factor 2.
