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. 2020 Feb 1;34(3-4):166–178. doi: 10.1101/gad.331157.119

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© 2020 Zhang et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Meiotic resumption defects caused by Snf2h deficiency can be rescued by restoring MPF activity. (A) Schematic illustration of the cAMP-PKA-MPF pathway. Red words with yellow background positively regulate cAMP-PKA activity, which leads to meiotic arrest at prophase I stage. Black words with gray background negatively regulate cAMP-PKA activity, which activates MPF and promotes meiotic resumption. Red words with no background are the strategies used for the rescue experiments. (B–F) GVBD ratio at different time points during IVM for oocytes injected with Prkar2b mRNA (B), Prkaca siRNA (C), Cdk1AF mRNA (D), Ccnb2 mRNA (E), or Ccnb1 mRNA (F). N = 3 independent biological replicates for each rescue experiment and n > 10 oocytes were used for each replicate. Data are presented as mean ± SEM.