FIG 5.
HPV16-induced changes in the transcriptome of cells grown in organotypic rafts. Viral transcript levels in undifferentiated (mono) and differentiated cells infected with WT and E7-TTL mutant viruses. RNA and DNA were collected from monolayer cells at 7 dpi and from 20-day-old rafts. Viral E6 transcript (A) and DNA (B) are expressed as ΔCT relative to the household gene controls cyclophilin A (A) and β-actin (B), respectively. (C) Detection of viral DNA in infected rafts by highly sensitive fluorescent in situ hybridization. (D) TPM values for viral transcripts in samples used for sequencing. (E) Numbers of genes whose expression was significantly modified in WT and E7-TTL HPV16-infected cells grown in organotypic raft cultures.