FIG 9.

p53, E6 protein, and viral genome in organotypic rafts. (A) Organotypic rafts derived from control (ctrl) and from WT and E7-TTL HPV16-infected HFK were paraffin embedded, sectioned, and processed for detection of p53 and viral DNA (vDNA) by immunofluorescent and fluorescent in situ hybridization, respectively. Adjacent sections were used for in situ hybridization and immunofluorescent staining. (B) Furthermore, E6 protein was detected using the OncoE6 cervical test. Note detection of p53 despite the presence of E6 protein and viral genome E7-TTL-harboring rafts.