TABLE 1.
Genes, primers, and annealing temperatures for qPCR
| Target genea | NCBI RSb | Primer sequencec | Annealing temp (°C) |
|---|---|---|---|
| Nav1.3 (h) | NM_006922.4 | AGACAGCGAAAGCAGGAGAG (f) | 58 |
| ACCCACCAAGGAAACCACAC (r) | |||
| Nav1.7 (h) | NM_002977.3 | TATGACCATGAATAACCCGC (f) | 58 |
| TCAGGTTTCCCATGAACAGC (r) | |||
| PPIA (h) | NM_005612.5 | AGCATACGGGTCCTGGCATCT (f) | 65 |
| CATGCTTGCCATCCAACCACTCA (r) | |||
| GFP | ACTTCAAGATCCGCCACAACA (f) | 58 | |
| TGATCGCGCTTCTCGTTGG (r) | |||
| ICP0 (v) | GACGGGCAATCAGCGGTTC (f) | 60 | |
| GTAGTCTGCGTCGTCCAGGT (r) | |||
| TK (v) | TACCCGAGCCGATGACTTAC (f) | 62 | |
| AAGGCATGCCCATTGTTATC (r) | |||
| LAT (v) | CGGCGACATCCTCCCCCTAAGC (f) | 60 | |
| GACAGACGAACGAAACGTTCCG (r) |
a
h, human; v, viral.
b
RS, reference single nucleotide polymorphism identification number.
c
f, forward primer; r, reverse primer.