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Interactions between the polymerase and ANP32 are localized in the nucleus. (a) Schematic of BiFC assay. Interaction of putative binding partners reconstitutes Venus, resulting in fluorescence. (b) HEK 293T cells were transfected with expression plasmids encoding PB1VN, PA, and either chANP32AVC or huANP32AVC in the presence or absence of PB2 627E. Twenty-four hours after transfection, cells were fixed and fluorescence was measured by confocal microscopy. Bars, 10 μm.
