TABLE 2.

Particle-to-FFU ratios for replication-competent mutants^a

Virus	No. of genome copies/ml	Fold change in no. of genome copies/ml relative to parent	No. of genome copies/TCID50
Parentb	1.4 × 106		1.2 × 103
Mutanthc	2.1 × 105	−6.80	2.5 × 103
Mutantmd	1.8 × 109	1,307	3.3 × 103
Mutantle	1.1 × 1010	7,852	4.6 × 103

^aHuh7.5 cells were transfected with transcripts of parent and mutant libraries. Replication-competent viruses were serially passaged. Infectious titers were determined by the TCID₅₀ method. Genome copies were measured by quantitative RT-PCR.

^bPassage 53 virus.

^cPassage 53 virus, derived from 10 ng 0.1A-0.2GCU library.

^dPassage 53 virus, derived from 10 ng 0.2AGCU library.

^ePassage 67 virus, derived from 50 ng 0.2AGCU library.