FIG 5.

K124/K128 and K139/K142 of the SFV capsid are critical for the dsRNA and siRNA binding. (A to C) MBP-capsid_WT, MBP-capsid_K124A/K128A, or MBP-capsid_K139A/K142A was incubated with 0.5 μM 200-nt DIG-labeled dsRNA (A), 1 μM 22-nt siRNA (B), or 0.5 μM ssRNA (C) at 25°C for 30 min. The complexes were separated on 1.5% native-TBE agarose gel, transferred to membranes, and then incubated with anti-DIG antibody conjugated with alkaline phosphatase. MBP-FB2 and MBP alone were used as positive and negative controls, respectively.