FIG 6.
Construction and recovery of VSR-deficient SFV. (A) SFV genome and the mutation sites of K124A/K128A and K139A/K142A. (B) Plaque morphology of SFVWT and SFVK124A/K128A. (C) The virions of SFVWT and SFVK124A/K128A were examined by TEM at magnifications of ×40,000. (D) 293T cells were infected with SFVWT and SFVK124A/K128A at an MOI of 5. At 15, 30, and 45 min postinfection, the viruses remaining in the supernatant and the viral RNAs entered into cells were examined by plaque assays and qRT-PCR, respectively. The RNA levels of SFVK124A/K128A in 293T cells at 15 min postinfection are defined as 1. All data represent the means and standard deviations of three independent experiments. n.s., not significant. (E) 293T cells were infected with SFVWT or SFVK124A/K128A (MOI = 0.1), respectively. Viral titers were measured at the indicated times using a standard plaque assay in Vero cells. All data represent the means and standard deviations of three independent experiments. *, P < 0.05; **, P < 0.01 (as measured by two-way analysis of variance [ANOVA]; GraphPad Prism).