TABLE 1.
Plasmids used in this study
| Name | Plasmid description | Template or source | Primer(s) | Enzymes or method |
|---|---|---|---|---|
| pYCD11 | pcDNA3.0-Flag-vector | pcDNA3.0 | LMRC1083, LMRC1084 | HindIII, KpnI |
| pcDNA3.0-Flag-BFLF2 | From Hsiu-Ming Shih (Academia Sinica, Taiwan) | |||
| pLSC12 | pcDNA3.0-Flag-BFLF2 D1 (Δ2-102) | pcDNA3.0-Flag-BFLF2 | LMRC876 | Mutagenesis |
| pYZS2 | pcDNA3.0-Flag-BFLF2 D2 (Δ103-149) | pcDNA3.0-Flag-BFLF2 | LMRC877 | Mutagenesis |
| pLSC15 | pcDNA3.0-Flag-BFLF2 D3 (Δ150-209) | pcDNA3.0-Flag-BFLF2 | LMRC878 | Mutagenesis |
| pYZS3 | pcDNA3.0-Flag-BFLF2 D4 (Δ209-276) | pcDNA3.0-Flag-BFLF2 | LMRC910 | Mutagenesis |
| pYZS4 | pcDNA3.0-Flag-BFLF2 D5 (Δ277-318) | pcDNA3.0-Flag-BFLF2 | LMRC880 | Mutagenesis |
| pYTL1 | pcDNA3.0-Flag-BFLF2 3A (R47,52A,K50A) | pcDNA3.0-Flag-BFLF2 | LMRC998 | Mutagenesis |
| pYCD12 | pcDNA3.0-Flag-BFLF2 dl (Δ2-27) | pcDNA3.0-Flag-BFLF2 | LMRC1006 | Mutagenesis |
| pYTL3 | pcDNA3.0-Flag-BFLF2 d2 (Δ29-57) | pcDNA3.0-Flag-BFLF2 | LMRC1007 | Mutagenesis |
| pYTL4 | pcDNA3.0-Flag-BFLF2 d3 (Δ59-80) | pcDNA3.0-Flag-BFLF2 | LMRC1008 | Mutagenesis |
| pYTL5 | pcDNA3.0-Flag-BFLF2 d4 (Δ82-106) | pcDNA3.0-Flag-BFLF2 | LMRC1009 | Mutagenesis |
| pYTL6 | pcDNA3.0-Flag-BFLF2 3Ad3 (R47,52A,K50A, Δ59-80) | pYTL4 | LMRC998 | Mutagenesis |
| pEYFP-C1-LacZ-NLS | From Mitsuhiro Kawata (Kyoto Prefectural University of Medicine, Japan) | |||
| pYCS2 | pEYFP-C1-LacZ | EcoRI, BamHI | ||
| pYCD5 | pEYFP-C1-LacZ-BFLF2 WT22–89 | pcDNA3.0-Flag-BFLF2 | LMRC1081, LMRC1082 | EcoRI, BamHI |
| pYCD6 | pEYFP-C1-LacZ-BFLF2 d222–89 (Δ29-57) | pcDNA3.0-Flag-BFLF2 d2 (Δ29-57) | LMRC1081, LMRC1082 | EcoRI, BamHI |
| pYCD7 | pEYFP-C1-LacZ-BFLF2 d322–89 (Δ59-80) | pcDNA3.0-Flag-BFLF2 d3 (Δ59-80) | LMRC1081, LMRC1082 | EcoRI, BamHI |
| pYCD8 | pEYFP-C1-LacZ-BFLF2 3A22–89 (R47,52A,K50A) | pcDNA3.0-Flag-BFLF2 3A (R47,52A,K50A) | LMRC1081, LMRC1082 | EcoRI, BamHI |
| pYCD9 | pEYFP-C1-LacZ-BFLF2 3Ad322–89 (R47,52A,K50A, Δ59-80) | pcDNA3.0-Flag-BFLF2 3Ad3 (R47,52A,K50A, Δ59-80) | LMRC1081, LMRC1082 | EcoRI, BamHI |
| pYCD10 | pcDNA3.0-HA-vector | pcDNA3.0 | LMRC1085, LMRC1086 | HindIII, KpnI |
| pcDNA3.0-HA-BFRF1 | From Hsiu-Ming, Shih (Academia Sinica, Taiwan) | |||
| pLPT4 | pcDNA3.0-HA-BFRF1 ΔLD1 | pcDNA3.0-HA-BFRF1 | LMRC833 | Mutagenesis |
| pLPT5 | pcDNA3.0-HA-BFRF1 ΔLD2 | pcDNA3.0-HA-BFRF1 | LMRC834 | Mutagenesis |
| pGTL1 | pGEX4T1-BFRF1 | pcDNA3.0-HA-BFRF1 | LMRC891, LMRC892 | Not I, BamHI |
| pGTL12 | pGEX4T1-BFRF1 d(72-313) | pGEX4T1-BFRF1 | LMRC932 | Mutagenesis |
| pGTL13 | pGEX4T1-BFRF1 d(135-313) | pGEX4T1-BFRF1 | LMRC933, LMRC935 | Mutagenesis |
| pYTJ1 | pGEX4T1-BFRF1 d(7-196) | pGEX4T1-BFRF1 | LMRC1062 | Mutagenesis |
| pYTJ2 | pGEX4T1-BFRF1 d(7-134) | pGEX4T1-BFRF1 | LMRC1063 | Mutagenesis |
| pYTJ3 | pGEX4T1-BFRF1 d(7-134, 201-313) | pGEX4T1-BFRF1 d(7-134) | LMRC1064 | Mutagenesis |