FIG 6.

Zta bZIP domain interaction with a ZRE and the NFAT/ARRE2 element. The ability of the GFP-tagged Zta bZIP domain to interact with the ARRE2 element was compared to that of a ZRE element by an electrophoretic mobility shift assay (EMSA). (A) DNA sequence of each probe, with the known ZRE (underscored), the NFAT element (bold), and the AP1 site (boxed) shown. (B) GFP and GFP-bZIP Zta proteins were produced, and 1 μg was analyzed by staining. (C) Interaction of each GFP and GFP-bZIP Zta protein with the indicated ZREs. The relative level of the complex is shown below each GFP-bZIP Zta lane.