FIG 8.

Proportions of defective HIV-1 DNA sequences from CD4⁺ T cell subsets during ART (AHI group). The effects of ART duration on the proportions of defective HIV-1 DNA p6-RT sequences in CD4⁺ T cell subsets sorted from PB (A to D), LNs (E to H), and gut (I to J) are indicated as OR per year on therapy. The 95% confidence intervals for the ORs are indicated in square brackets. Estimated by mixed-effects logistic regression, the fitted curves (red lines) follow the following equation: y = 100 × {1 + exp[−(k + xs)]}⁻¹, where y represents the proportions of defective HIV-1 DNA p6-RT sequences, k represents the y intercept, s represents the coefficient calculated as log(OR), and x represents the years on ART. Each HIV-1 DNA sequence is the unit of analysis, and the denominator for each sequence is a single HIV-1-infected T cell (33, 73). The confidence interval of each data point was derived from the binomial distribution. The odds ratios and their confidence intervals were derived by logistic regression models. Solid blue diamonds, T_N cells; solid blue triangles, T_CM cells; solid blue circles, T_TM cells; solid blue squares, T_EM cells, all derived from PB. Solid pink diamonds, T_N cells; solid pink triangles, T_CM cells; solid pink circles, T_TM cells; solid pink squares, T_EM cells, all derived from LNs. Solid green triangles, T_CTM cells; solid green squares, T_EM cells, all derived from the gut.