. 2020 Jan 17;94(3):e01270-19. doi: 10.1128/JVI.01270-19

TABLE 3.

Cellular markers used for a broad spectrum of CD4⁺ T cell subsets sorted from PB, LNs, and gut

Anatomic site	CD4⁺ T cell subset	Cell markers	Pt IDs^a
PB	T_N	CD3⁺ CD4⁺ CD45RO⁻ CCR7⁺ CD27⁺ CD57⁻	2243, 2302, 2303, 2277, 2370, 2443, 2268, 2278, 2259, 2286, 2452, 1292, 2470, 2467, 2469, 2254, 2241, 2445, 2450, 2472
	T_N	CD3⁺ CD4⁺ CD45RA⁺ CD45RO⁻ CCR7⁺ CD27⁺ CD127⁺ CD95⁻	2275, 2115, 2518, 2046, 2013, 2026
	T_SCM	CD3⁺ CD4⁺ CD45RA⁺ CD45RO⁻ CCR7⁺ CD27⁺ CD127⁺ CD95⁺	2275, 2115, 2518, 2046, 2013, 2026
	T_CM	CD3⁺ CD4⁺ CD45RO⁺ CCR7⁺ CD27⁺	2243, 2302, 2303, 2277, 2370, 2443, 2268, 2278, 2259, 2286, 2452, 1292, 2470, 2467, 2469, 2254, 2241, 2445, 2450, 2472
	T_CM	CD3⁺ CD4⁺ CD45RA⁻ CD45RO⁺ CCR7⁺ CD27⁺	2275, 2115, 2518, 2046, 2013, 2026
	T_TM	CD3⁺ CD4⁺ CD45RO⁺ CCR7⁻ CD27⁺	2243, 2302, 2303, 2277, 2370, 2443, 2268, 2278, 2259, 2286, 2452, 1292, 2470, 2467, 2469, 2254, 2241, 2445, 2450, 2472
	T_TM	CD3⁺ CD4⁺ CD45RA⁻ CD45RO⁺ CCR7⁻ CD27⁺	2275, 2115, 2518, 2046, 2013, 2026
	T_EM	CD3⁺ CD4⁺ CD45RO⁺ CCR7⁻ CD27⁻	2243, 2302, 2303, 2277, 2370, 2443, 2268, 2278, 2259, 2286, 2452, 1292, 2470, 2467, 2469, 2254, 2241, 2445, 2450, 2472
	T_EM	CD3⁺ CD4⁺ CD45RA⁻ CD45RO⁺ CCR7⁻ CD27⁻	2275, 2115, 2518, 2046, 2013, 2026
	X5⁺ R6⁻	CD3⁺ CD4⁺ CD45RA⁻ CD45RO⁺ CXCR5⁺ CCR6⁻	2275, 2115, 2518, 2046, 2013, 2026
	X5⁻ R6⁺	CD3⁺ CD4⁺ CD45RA⁻ CD45RO⁺ CXCR5⁻ CCR6⁺	2275, 2115, 2518, 2046, 2013, 2026
LNs	T_N	CD3⁺ CD4⁺ CD45RO⁻ CCR7⁺ CD27⁺ CD57⁻	2303, 2370, 2443, 2268, 2452, 1292, 2470, 2467, 2469, 2241, 2445, 2472
	T_CM	CD3⁺ CD4⁺ CD45RO⁺ CCR7⁺ CD27⁺	2303, 2370, 2443, 2268, 2452, 1292, 2470, 2467, 2469, 2241, 2445, 2472
	T_TM	CD3⁺ CD4⁺ CD45RO⁺ CCR7⁻ CD27⁺	2303, 2370, 2443, 2268, 2452, 1292, 2470, 2467, 2469, 2241, 2445, 2472
	T_EM	CD3⁺ CD4⁺ CD45RO⁺ CCR7⁻ CD27⁻	2303, 2370, 2443, 2268, 2452, 1292, 2470, 2467, 2469, 2241, 2445, 2472
Gut	T_N	CD3⁺ CD4⁺ CD45RO⁻ CD27⁺	2302, 2277, 2370, 2443, 2268, 2259, 2286, 2275, 2452, 2254, 2241, 2445
	T_CTM	CD3⁺ CD4⁺ CD45RO⁺ CD27⁺	2302, 2277, 2370, 2443, 2268, 2259, 2286, 2275, 2452, 2254, 2241, 2445
	T_EM	CD3⁺ CD4⁺ CD45RO⁺ CD27⁻	2302, 2277, 2370, 2443, 2268, 2259, 2286, 2275, 2452, 2254, 2241, 2445
	CD4⁺	CD3⁺ CD4⁺ CD8⁻ CD14⁻	2115, 2518, 2046, 2013, 2026

Identifiers (IDs) of participants included in the particular cell sorting.