HIV Tat reverses the inhibition of motility, invasion, and tube formation by LINC00313. (A) The level of LINC00313 in HMEC1, HUVEC, and SLK cells was determined by RT-qPCR. (B) HMEC1 and HUVEC were transduced with control or lentivirus-expressing LINC00313. At 24 h after transduction, cells were treated with or without HIV Tat for another 48 h. Successful overexpression of LINC00313 in HMEC1 and HUVEC was determined by RT-qPCR. (C, E, and G) Representative images from cell migration (C), invasion (E), and tube formation (G) assays of HMEC1 cells (upper panel) and HUVECs (lower panel) treated as described for panel B. (D, F, and H) Quantification of cell migration (D), invasion (F), and tube formation (H) of HMEC1 cells (left panel) and HUVECs (right panel) as described for panels C, E, and G, respectively. Data shown are means ± SD (n = 3). ***, P < 0.005 (Student’s t test).