FIG 7.

LINC00313 interacted with EZH2, and HIV Tat reduced this association. (A) An RNA pulldown assay was performed by incubation of in vitro-transcribed biotin-labeled LINC00313 with TCLs prepared from SLK cells transfected with pcDNA3-Flag-EED, pcDNA3-Flag-EZH2, and pcDNA3-Flag-SUZ12 with or without pcDNA3-HA-HIV Tat. Products pulled down by streptavidin beads were examined by immunoblotting using anti-Flag and anti-HA antibodies. (B) Expression levels of CRHBP and TP63 in HMEC1 cells treated as described in the legend to Fig. 5B were determined by RT-qPCR.