Table 1.

Quantification of Thiolated Conjugates from Mixed Thiol Incubation Experiments with Compound 1^a

sample	MA	[MA + BME]	[MA + MPA]
200 μM 1	100%	n/a	n/a
1 + 100 mM βME	12.14%	87.86%	n/a
1 + 100 mM MPA	6.49%	n/a	93.51%
1 + 50 mM βME, then 50 mM MPA	8.23%	30.61%	61.16%
1 + 50 mM MPA, then 50 mM βME	7.46%	29.84%	62.70%
1 + 100 μM βME	98.30%	1.70%	n/a
1 + 100 μM MPA	97.14%	n/a	2.86%
1 + 50 μM βME, then 50 μM MPA	97.50%	0.87%	1.63%
1 + 50 μM MPA, then 50 mM βME	97.79%	0.75%	1.46%

^aTable entries are relative values from integration of M + H extracted ion chromatograms.

MA = Michael acceptor. MA M + H = 307; [MA+βME] M + H = 385; [MA + MPA] M + H = 413.